Regulatory

Part:BBa_K4436690:Design

Designed by: Vedanta Kompella   Group: iGEM22_UIUC_Illinois   (2022-10-12)


narg CC(-40.5) Promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 193
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We simply followed the design laid out by Hothersall et al. when they designed the promoter.


Source

The promoter is a combination of the narG and CRP promoter, both native to E. coli.

References